New study contradicts assumption that true frogs diversified as they expanded their range around globe

Evolutionary biologists long have supposed that when species colonize new geographic regions they often develop new traits and adaptations to deal with their fresh surroundings. They branch from their ancestors and multiply in numbers of species.

Ranidae family are most diverse frog group in the world, found on all the world's continents except Antarctica 
[Credit: Chan Kin Onn]

Apparently, this isn't the story of "true frogs." The frog family scientists call Ranidae are found nearly everywhere in the world, and their family includes familiar amphibians like the American Bullfrog and the European common frog.

New research from the University of Kansas appearing in Royal Society Biology Letters shows, in contrast to expectations, "the rapid global range expansion of true frogs was not associated with increased net-diversification."

"First, we had to identify where these true frogs came from and when they started their dispersal all over the world," said lead author Chan Kin Onn, a doctoral student at KU's Biodiversity Institute. "We found a distinct pattern. The origin of these frogs was Indochina -- on the map today, it's most of mainland Asia, including Thailand, Vietnam, Cambodia and Burma. True frogs dispersed throughout every continent except Antarctica from there. That's not a new idea. But we found that a lot of this dispersal happened during a short period of time -- it was during the late Eocene, about 40 million years ago. That hadn't really been identified, until now."

Next, Chan and co-author Rafe Brown, curator-in-charge of the KU Biodiversity Institute's Herpetology Division, looked to see if this rapid dispersal of true frogs worldwide triggered a matching eruption of speciation.

To establish the actual timing of true frogs' diversification, Chan and Brown performed phylogenetic analysis of 402 genetic 
samples obtained from an online database called GenBank. These samples represented 292 of the known 380 true 
frog species in the world [Credit: Rafe Brown]

"That was our expectation," Chan said. "We thought they'd take off into all this new habitat and resources, with no competition -- and boom, you'd have a lot of new species. But we found the exact opposite was true. In most of the groups, nothing happened. There was no increase in speciation. In one of the groups, diversification significantly slowed down. That was the reverse of what was expected."

To establish the actual timing of true frogs' diversification, Chan and Brown performed phylogenetic analysis of 402 genetic samples obtained from an online database called GenBank. These samples represented 292 of the known 380 true frog species in the world.

"We mined all of these sequences and combined them into a giant analysis of the whole family," Chain said. "It is to my knowledge the most comprehensive Ranidae phylogenetic analysis ever performed that included most of the representative species from the family."

Chan and Brown focused on four genes that would help to establish the family tree of true frogs.

"It's a genealogical pedigree of specimens, a family tree of species," Chan said. "Normally, you think of family tree as everyone in one family and how the various people are related. But this is more expanded where we look at how species are related to each other, so you can trace ancestry back in time."

The researchers concluded true frogs didn't become one of the most biodiverse frog family due to dispersing 
into new ranges, or due to filling a gap created by a catastrophic die-off. Rather, the rich diversity of species 
in the Ranidae family comes from millions of years' worth of continual evolution influenced by 
a host of different environs [Credit: University of Kansas]

After completing the phylogenetic analysis, the KU researchers used several frog fossils to "time calibrate" the history of the frogs' global dispersal.

"We use fossil frogs because we can accurately date the fossils," Chan said. "We know we found the fossil in a certain rock deposit, and we know with confidence how old the deposit is, so then we can estimate the age of the fossil."

After Chan and Brown deduced similarities between fossilized true frogs as reported by paleontologists and contemporary true frogs, they placed fossils into groups of closely related species, which scientists call genera.

"Using data from paleontological studies, we can loosely place a fossil where in the phylogeny it belongs and can put a time stamp on that point," Chan said. "That's where calibration happens, each fossil is sort of like an anchor point. You can imagine with a really big phylogeny, the more anchor points or calibration points the better your time estimate."

Through this process, the KU researchers concluded true frogs didn't become one of the most biodiverse frog families due to dispersing into new ranges, or due to filling a gap created by a catastrophic die-off (such as the Eocene-Oligocene Extinction Event that triggered widespread extinctions from marine invertebrates to mammals in Asia and Europe).

Rather, the rich diversity of species in the Ranidae family comes from millions of years' worth of continual evolution influenced by a host of different environs.

"Our conclusion is kind of anticlimactic, but it's cool because it goes against expectations," Chan said. "We show the reason for species richness was just a really steady accumulation of species through time -- there wasn't a big event that caused this family to diversify like crazy."

Source: University of Kansas [September 13, 2017]